Effect of Ethanol Extract and Fractions of Physalis Calyx Seu Fructus on Inflammation and Mycoplasma Gallisepticum

The aim of the study was to investigate whether ethanol extract and fractions from D-101 macroporous resin of Physalis Calyx Seu Fructus has the anti-inflammatory and anti-Mycoplasma gallisepticum (MG) activity in vitro. Extract (P) was isolated from the dried calyces of Physalis Calyx Seu Fructus with 80% ethanol under reflux. The five major fractions (P1, P2, P3, P4 and P5) were obtained with ethanol gradient elution from D-101 macroporous resin column loaded with P. P and Fraction P2 was enriched with luteoloside content, with 1.39% and 2.548%, respectively. P3 had significantly inhibited the expression of IL-6, TNF-alpha and NO both at protein and gene level in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages by the qRT-PCR analysis and ELISA assay. Moreover, P2, P3, and P4 at its minimum inhibitory concentration inhibited the growth of MG. Conclusively, the present study suggested that P3 is a potential agent against MG and inflammation. (C) 2020 PVJ. All rights reserved