A novel automatic segmentation and tracking method to measure cellular dielectrophoretic mobility from individual cell trajectories for high throughput assay

•Automatic segmentation and tracking method to measure DEP-induced trajectories of numerous cells at the single cell level.•Evaluation of the method with cell count capability, cell trapping efficiency, and cell trapping Occupancy.•Measurement of dielectric mobility of each cell using the automatic method in a robust, efficient, and accurate manner.•Verification in comparison of the measured cellular dielectric mobility with previous results and statistical consistency.