Antimicrobial Activity and Phytochemical Screening of Artemisia annua L . and Millotus philippensis ( Lam . ) Mull . Arg . leaves

The phytochemical screening of ethanolic extract of leaves of Artemisia annua L. and Millotus philippensis (Lam.) Mull. Arg. And their Antimicrobial activity were screened through by Disc diffusion assays against seven bacterial strains; (four Gram negative bacteria, three Gram positive bacteria) and one fungal strain Candida albican. Two concentrations (6 mg/ml and 12 mg/ml) were used to check the antimicrobial activity of plant extracts. The result showed that Millotus philippensis (Lam.) Mull. Arg leaves extract showed best activity against all tested microorganism at all concentration except S. typhi, while Artemisia annua L., were more active against gram positive bacteria but no activity was observed against Salmonila typhi, Kleibsiella pneumonia and fungal strain Candida albican. The phytochemical screening revealed the presence of secondary metabolites alkaliod, tanine, saponine and terpeniods in varying concentrations. This research supports the use of the leaves of Artemisia annua L and Millotus philippensis (Lam.) Mull. Arg for prophylactic and therapeutic purpose against bacterial and fungal infection.