Anaerobic digestion of spectinomycin mycelial residues pretreated by thermal hydrolysis: removal of spectinomycin and enhancement of biogas production

Anaerobic digestion (AD) is an effective technology to dispose antibiotic mycelial residues, but biogas production is influenced by hydrolysis rates and antibiotic residue. Herein, the effects of thermal hydrolysis pretreatment for AD of spectinomycin mycelial residues (SMRs) were investigated. The results showed that the removal ratio of spectinomycin was increased while the temperature of pretreatment was escalating. Meanwhile, thermal hydrolysis facilitated the dissolution of organic matters. However, non-biodegradable substances measured by fluorescence excitation–emission matrix accumulated and thus had an adverse influence on biogas production. Based on batch assays, the optimal pretreatment temperature for SMRs was 120 °C. The removal of spectinomycin was benefit for biogas production (increasing by 7.6%), and the overall biogas production increased by 27.6% compared with 289.90 mL gVS −1 of untreated SMRs. The microbial community analysis revealed that spectinomycin (265 mg L −1 ) might influence bacteria in the early stage of AD (first 5 days), while redundancy analysis showed that spectinomycin had a non-significant influence on community succession over the 32 days of fermentation.