Systemic Immune Response Profiling With Sylaras Implicates A Role For Cd45r/B220(+) Cd8(+) T Cells In Glioblastoma Immunology.

Research tools for the rapid and accurate assessment of bulky single-cell datasets are needed to achieve a more integrated comprehension of immune response to disease and therapy. Here we describe SYLARAS (Systemic Lymphoid Architecture Response Assessment), a modular and extensible research platform for the acquisition, statistical analysis, and visual display of multi-organ immune response data. Leveraging our technology against a syngeneic mouse model of glioblastoma (GBM), we reveal the tumor’s influence on immune cell subsets hitherto undescribed in the disease, including CD45R/B220+ CD8+ T cells characterized by their ability to constrain immune response to autologous antigens. Ancillary studies show that these cells are transcriptionally and morphologically distinguishable from conventional CD8+ T lymphocytes and infiltrate the brain tumor microenvironment of rodents and humans. The ability of SYLARAS to provide ample content on the cell and molecular mechanisms governing systemic immune response makes it a broadly useful preclinical research tool in the era of immunotherapy. Citation Format: Gregory J. Baker, Sucheendra K. Palaniappan, Jodene K. Moore, Stephanie H. Davis, Sandro Santagata, Peter K. Sorger. Systemic immune response profiling with SYLARAS implicates a role for CD45R/B220+ CD8+ T cells in glioblastoma immunology [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology and Immunotherapy; 2019 Nov 17-20; Boston, MA. Philadelphia (PA): AACR; Cancer Immunol Res 2020;8(3 Suppl):Abstract nr A9.