Effect and mechanism of CyPA in radiosensitization of lung adenocarcinoma using CRISPR/Cas9 technology

Abstract Objective To explore the role of cyclophilin A (CyPA) in sensitization of lung adenocarcinoma to radiotherapy using CRISPR/Cas9 technology. Methods A CyPA knockout human lung adenocarcinoma cell line H1975 was established by CRISPR/Cas9 technology. Groups included a control group (wildtype), CyPA knockout group, traditional Chinese medicine (TCM) extract with Fuzhengzengxiao decoction group, and TCM extract with Fuzhengzengxiao decoction + CyPA knockout group. Each group was exposed to radiation at doses of 0, 2, 4, 6, and 8 Gy. After 24 hours, MTT assays were used to determine the survival rate of lung cancer cells and calculate radiosensitivity. The qPCR was used to measure mRNA expression of DDIT3, CDKN1A, and CDC25A associated with DNA damage repair. Results Without irradiation, Fuzhengzengxiao decoction reduced the survival rate of lung adenocarcinoma cells (P Conclusion Fuzhengzengxiao decoction significantly decreases the survival rate of lung cancer cells, and its mechanism may be related to radiosensitization by decreasing expression of CyPA and inducing G1/S cell cycle arrest.