Immunopeptidome screening to design An immunogenic construct against PRAME positive breast cancer; An in silico study

•Investigating and validation of PRAME co-expressed genes.•Establishing protein-protein interaction network of PRAME and enrichment analysis.•Design novel multi-epitope BC vaccine against PRAME.•Prediction and modeling of secondary and tertiary structures of PRAME derived vaccine.•In-silico validation of the designed construct by studying modelled tertiary structure, physiochemical characterization, safety, antigenicity, post-translational modification, solubility, and docking simulation.