Three-sectioning method: A procedure for studying hard tissues and large pieces under light and electron microscopy.

•The serial sections are easy to manipulate as a whole and artifact-free.•Suitable to study the interphase prostheses-tissues.•To cut areas with different hardness without causing splintering or wrinkling.•The avoidance of grinding saves most of the original piece.•Useful for stereo, light and electron microscopes.