Fe/C magnetic nanocubes with enhanced peroxidase mimetic activity for colorimetric determination of hydrogen peroxide and glucose

Iron-carbon (Fe/C) magnetic nanocubes were synthesized by direct pyrolysis of Prussian blue nanocubes under an inert gas atmosphere. They are shown to possess intrinsic peroxidase mimicking activity to catalyze the oxidation of peroxidase substrate N , N -diethyl- p -phenylenediamine sulfate salt to form a purple colored product in the presence of H 2 O 2 . The values for K m and V max are 74 μM and 46 nmol s −1 , respectively. Steady-state kinetic analysis also indicates that the catalysis reaction follows a ping-pong mechanism. Based on these findings, an ultrasensitive colorimetric H 2 O 2 assay was worked. Absorbance (best value measured at 550 nm) increases linearly in the 10 nM to 0.2 mM H 2 O 2 concentration range, and the limit of detection is 1.5 nM. The method was also applied to the quantification of glucose, which is oxidized by glucose oxidase in the coexistence of H 2 O 2 . The response covers the 0.1 to 500 μM glucose concentration range, and the limit of detection is 16 nM. The method was applied to the determination of H 2 O 2 in rainwater samples. The glucose assay was used to analyze serum samples, and satisfactory results were obtained. Other attractive features include good chemical activity, low cost, easy storage, and high catalytic efficiency. Graphical abstract Schematic presentation of converting Prussian blue nanoparticles into Fe/C magnetic nanocubes by a pyrolysis technique and the use of glucose oxidase and Fe/C magnetic nanocubes to establish a one-step spectrophotometric method for the determination of glucose and hydrogen peroxide.