Real-Time Monitoring Of T-Cell-Secreted Interferon-Gamma For The Diagnosis Of Tuberculosis

In this paper, we report the development of a label-free biosensor, based on surface plasmon resonance, for real-time monitoring of captured human CD4(+)T-cells, and their dynamic cytokine production upon specific antigen stimulation. Microarrays of CD4(+)T-cells, and interferon gamma (IFN-gamma) specific antibody (Ab) spots were printed side by side onto the poly(OEGMA-co-HEMA) matrix. The placement of CD4(+)T-cells near the anti IFN-gamma Ab-coated spots ensured a high local concentration of secreted IFN-gamma for detection. We have demonstrated that this approach enables the detection of tuberculosis (TB) infection in clinical samples, with an overall sensitivity of 85.5% and an overall specificity of 97.7%.