First draft genome assembly of the Argane tree ( Argania spinosa )

F1000Research(2018)

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摘要
The Argane tree ( L. Skeels) is an endemic tree of southwestern Morocco that plays an important socioeconomic and ecologic role for a dense human population in an arid zone. Several studies confirmed the importance of this species as a food and feed source and as a resource for both pharmaceutical and cosmetic compounds. Unfortunately, the argane tree ecosystem is facing significant threats from environmental changes (global warming, over-population) and over-exploitation. Limited research has been conducted, however, on argane tree genetics and genomics, which hinders its conservation and genetic improvement. Here, we present a draft genome assembly of . A reliable reference genome of   was created using a hybrid  assembly approach combining short and long sequencing reads. In total, 144 Gb Illumina HiSeq reads and 7.2 Gb PacBio reads were produced and assembled. The final draft genome comprises 75 327 scaffolds totaling 671 Mb with an N50 of 49 916 kb. The draft assembly is close to the genome size estimated by -mers distribution and covers 89% of complete and 4.3 % of partial orthologous groups in BUSCO. The genome will be useful for assessing biodiversity leading to efficient conservation of this endangered endemic tree. Furthermore, the genome may enable genome-assisted cultivar breeding, and provide a better understanding of important metabolic pathways and their underlying genes for both cosmetic and pharmacological purposes.
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关键词
Argane,Argania spinosa,Assembly,Endemic,Genome,International Argane Genome Consortium,Morocco
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