Optimizing the 3D-reconstruction technique for serial block-face scanning electron microscopy

Stefan Wernitznig,Mariella Sele,Martin Urschler,Armin Zankel,Peter Pölt,F. Claire Rind,Gerd Leitinger

Journal of Neuroscience Methods（2016）

引用 6|浏览1

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摘要

•We describe a straightforward method to segment cells in SEM images.•With the new software the segmentation is much faster than manual drawing.•The segmentation output can be transferred to a variety of visualization tools.

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关键词

Locust,Serial block-face scanning electron microscopy,Semi-automatic segmentation,3D-reconstruction

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