Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y O and N types in potato.

•Primers targeting the highly conserved pipo region specifically recognized different Potato virus Y isolates by RPA.•The RPA assay efficiently detects viral RNA in low viral titer samples, including the peridermal tissues of potato tubers.•The RPA procedure was streamlined by using crude extract from infected-potato tissues.•The real-time detection of Potato virus Y using fluorescent probe picked up the viral RNA within 6 min.