Method to Quantify Cytokines and Chemokines in Mouse Brain Tissue Using Bio-Plex Multiplex Immunoassays.

•Multiplex ELISA platforms can measure various protein quantities from a single, small volume of sample. Here, we describe the analysis of 33 different cytokines and chemokines from inflamed brain tissue harvested from mice infected with the parasite Plasmodium berghei – a pathogen that induces a disease referred to as cerebral malaria.