A novel image segmentation method for the evaluation of inflammation-induced cortical and hippocampal white matter injury in neonatal mice.

•A novel image segmentation method for quantification of myelinated fibers in brain sections.•The developed algorithm converted to a program called” MyelinQ”.•The program is automated, consistent and easy-to-use.•The method revealed that a viral mimic causes significant hypomyelination in neonatal mouse brain.