Novosphingobium Clariflavum Sp Nov., Isolated From A Household Product Plant

A Gram-stain-negative, rod-shaped, bright-yellow-pigmented bacterium, designated 164(T), was isolated from a used sponge for equipment cleaning at a household product plant in China. The 16S rRNA gene sequence comparisons indicated that strain 164(T) was most closely related to Novosphingobium panipatense DSM 22890(T) (98.28% similarity) and shared sequence similarities of 97.73-98.27% with other members of the genus Novosphingobium. In DNA-DNA hybridization studies the relatedness between strain 164(T) and its closest phylogenetic neighbours was < 70 %, which indicated that strain 164(T) represented a novel species of the genus Novosphingobium. The DNA G+C content of strain 164(T) was 65.9 mol%. The major respiratory quinone was ubiquinone Q-10 (83.5 %) with minor amounts of Q-9 (16.5 %). The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine, unidentified aminolipids and unidentified aminophospholipids. Spermidine was the major polyamine. The major fatty acids were summed feature 8 (consisting of C-18 : 1 omega 7c and/or C-18 : 1 omega 6c) and C-14 : 0 2-OH. The results obtained from phylogenetic analysis, DNA-DNA hybridization, and chemotaxonomic and phenotypic analysis support the conclusion that strain 164(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium clariflavum sp. nov. is proposed. The type strain is 164(T) (=CICC 11035s(T) =DSM 103351(T)).