Algoriphagus Resistens Sp Nov., Isolated From Marine Sediment

Strain NH1(T), a pink-pigmented, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative, Gram-stainnegative marine bacterium, was isolated from marine sediment on the coast of Weihai, China. Cells of strain NH1T were rodshaped, 0.8-2.0 mu m in length and 0.5-1.0 mu m in width. The strain was able to grow at 13-37 degrees C, pH 5.5-8.5, in the presence of 0.0-8.0% (w/v) NaCl. Optimal growth was observed at 28 degrees C, with 3.0% (w/v) NaCl and pH 6.5-7.0. Nitrate was reduced. The G+C content of the DNA was 41.9 mol%. The major isoprenoid quinone was MK-7 and the main cellular fatty acids (>10 %) were summed feature 3 (33.6 %) comprising iso-C-15 : 0 2-OH and/or C-16: (1)omega 7c, and iso-C-15: 0 (19.2%). The major polar lipids in strain NH1(T) were phosphatidylethanolamine, unidentified lipids, phospholipid and aminolipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NH1(T) was highly related to the type strains of Algoriphagus antarcticus (97.87% 16SrRNA gene sequence similarity) and Algoriphagus ratkowskyi (97.56 %). On basis of the phenotypic and phylogenetic data, strain NH1(T) should be classified as representing a novel species of the genus Algoriphagus, for which the name Algoriphagus resistens sp. nov. is proposed. The type strain is NH1(T) (= MCCC 1H00140(T) = KCTC 52228(T)).