Heifers express G-protein coupled receptor 61 in anterior pituitary gonadotrophs in stage-dependent manner.

•Majority of GPR61 colocalized with GnRHR in the plasma membrane of gonadotrophs.•GPR61 levels were lowest during early luteal phase of the reproductive cycle.•GPR61 gene 5′-flanking region contains estrogen/progesterone-type response elements.•GPR61 may mediate reproductive stage-specific signaling in bovine anterior pituitary.