Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq

NATURE PROTOCOLS(2017)

引用 98|浏览73
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摘要
This protocol describes how to integrate whole-cell patch-clamp in single neurons from mouse brain tissue slices with single-cell RNA sequencing and morphological recovery.
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关键词
Computational biology and bioinformatics,Immunohistochemistry,Neuroscience,Patch clamp,RNA sequencing,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry
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