Toxoplasma gondii-positive human sera recognise intracellular tachyzoites and bradyzoites with diverse patterns of immunoreactivity

•We screened anti-Toxoplasma gondii IgG+  or IgM+ human sera for detection of additional antigens.•Anti-T. gondii IgG+ sera showed immune reactivity to intracellular tachyzoites.•The IgG+ sera were also immune-reactive to in vitro induced bradyzoites.•Immune reactivity to in vitro bradyzoites was not observed in IgM+/IgG− sera.•Future investigations of these antigens are needed to demonstrate clinical utility.