Assessment of census ( N ) and effective population size ( N e ) reveals consistency of N e single-sample estimators and a high N e / N ratio in an urban and isolated population of fire salamanders

mphibians are the most threatened vertebrates on Earth, and one of the main factors involved in their decline is the loss and fragmentation of their natural habitats. Contemporary urban development is a major cause of habitat fragmentation, and populations trapped within urban environments offer a unique opportunity to study effects of fragmentation. Here, we compared, for the first time in fire salamanders ( Salamandra salamandra ), estimates of census ( N ) and effective population size ( N e ) in a small urban population in the city of Oviedo (Spain). We performed a 4 year capture-mark-recapture study and used three single-sample N e estimators based on 58 individuals genotyped for 15 polymorphic microsatellite loci. Our study showed a small ( N̂ = 113 salamanders; 95