Solvent-free peptide synthesis assisted by microwave irradiation: environmentally benign synthesis of bioactive peptides

An efficient and facile, solvent-free peptide synthesis assisted by microwave irradiation, using DIC/HONB as the coupling reagent combination is reported. Key features of this original protocol are solvent-free synthesis, very short reaction time and scalability without affecting yield and purity. The versatility of the method was successfully demonstrated by synthesizing several biologically active peptides in high purity, yield and without racemization.