Isolation and characterization of a new less-toxic derivative of the Fusarium mycotoxin diacetoxyscirpenol after thermal treatment.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY(2011)
摘要
Trichothecenes are an important class of mycotoxins that act as potent protein synthesis inhibitors in eukaryotic organisms. The compound 4,15-diacetoxyscirpenol is highly toxic for plants and animals. Potatoes are especially prone to be contaminated with 4,15-diacetoxyscirpenol after infection with Fusarium sambucinum. In the current study, the reduction of 4,15-diacetoxyscirpenol during thermal treatment in aqueous solution was monitored. A new derivative was detected and named DASM I. After isolation, DAS-M1 was characterized with LC-HR-MS and LC-MS/MS and structurally elucidated with H-1, C-13, and 2D NMIR Potatoes were inoculated with F. sambucinum, and the infected potatoes were cooked at 100 or 121 degrees C, respectively. A reduction of 4,15-diacetoxyscirpenol from about 26% (1 h at 100 degrees C) to 100% (4 h at 121 degrees C) was detected by means of LC-MS/MS analysis. The effects of different pH values on the reduction of 4,15-diacetoxyscirpenol were investigated, showing higher conversion rates at more acidic pH values. In addition, the toxicity of 4,15-diacetoxyscirpenol and DAS-M1 was compared in vitro using a wheat germ transcription/translation assay and in vivo on Saccharomyces cerevisiae. The results show that the inhibitory effect of DAS-M1 on yeast growth is about 50 times lower and inhibition of protein synthesis is about 100 times lower than that of 4,15-diacetoxyscirpenol.
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关键词
diacetoxyscirpenol,trichothecene,mycotoxin,detoxification,potatoes,Fusarium
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