Changed-transcriptional activity of retrotansposons induced by implantation of low-energy ion beam effected the expression of genes adjacent to retrotransposons

In order to study the expression profiles of the retrotransposons in rice implanted by low-energy ion beam and the effects on adjacent genes to these retrotransposons, we analyzed expression features of the retrotransposons in rice with exposure to the N+ ion beam implantation (6x10(17) N+/cm(2)), using the Agilent Rice Oligo Microarray (4x44K) Genome Array. The results showed that there were 43 probe sets in chip, 4 out of these transcripts were up or down-regulated (>= 2fold), including the gag, pol, and int. These four transcripts were heterogeneous to the other members in the family by clustering analysis. We also found that this differential expression effects the genes expression were up 1MB to down 1MB from the differentially expressed retrotranscription ESTs, representing the same up or down regulated case. These findings suggested that retrotransposons in rice were related to the response to N+ ion-beam implantation through the regulation of their adjacent genes. [LI Yong-hui, YU Xiang-li, LI Ya-he, GUO Xiang-meng, YA Hui-yuan. Changed-transcriptional activity of retrotansposons induced by implantation of low-energy ion beam effected the expression of genes adjacent to retrotransposons. Life Science Journal. 2011; 8(4):575-579] (ISSN: 1097-8135). http://www.lifesciencesite.com.