Digestip: A New Device For A Rapid And Efficient In-Solution Protein Digestion
Proteomics(2008)
摘要
DigesTip is a new device for in-solution protein digestion, based on a patent pending technology, able to immobilize enzymes (trypsin, in this case) on a solid surface, keeping their activity preserved. DigesTip is a standard pipette tip, usable both by human and by robots. Its main performances are: very short digestion time (1 min) and usability with low protein sample concentrations (5 mu g/mL). DigesTip obtains a clear signal in MS measurements and its usage rules out several preparative steps.
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关键词
fragment coverage,immobilized trypsin,mass spectrometry,protein digestion
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