Quantification of the yeast transcriptome by single-molecule sequencing

Doron Lipson,Tal Raz,Alix Kieu,Daniel R Jones,Eldar Giladi,Edward Thayer,John F Thompson,Stan Letovsky,Patrice Milos,Marie Causey

NATURE BIOTECHNOLOGY（2009）

引用 181|浏览26

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摘要

Lipson et al . profile the yeast transcriptome using single-molecule sequencing. This approach avoids the inherent biases of the digestion, ligation and amplification steps in alternative methods based on microarrays or other sequencing technologies.

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关键词

sample preparation,genetic analysis,gene expression,high throughput,biotechnology,reverse transcription,nature

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